Effect of equilibration times, freezing, and thawing rates on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa
نویسندگان
چکیده
منابع مشابه
Effect of Extender and Equilibration Time on Post Thaw Motility and Chromatin Structure of Buffalo Bull (Bubalus Bubalis) Spermatozoa
OBJECTIVE The aim of the present study was to investigate the effects of four equilibration times (2, 4, 8 and 16 hours) and two extenders (tris or Bioxcell®) on cryopreservation of buffalo semen. MATERIALS AND METHODS In this experimental study, split pooled ejaculates (n=4), possessing more than 70% visual sperm motility were divided in two aliquots and diluted in Bioxcell® and tris-citric ...
متن کاملEffect of Different Thawing Rates on Post-Thaw Viability, Kinematic Parameters and Chromatin Structure of Buffalo (Bubalus bubalis) Spermatozoa
OBJECTIVE The aim of the present study was to evaluate three thawing rates on the post thaw motility, viability and chromatin structure of buffalo semen frozen in 0.5-ml straws. MATERIALS AND METHODS In this experimental study semen was collected with artificial vagina (42℃) from four buffalo bulls.Split pooled ejaculates (n=4) were extended at 37℃ with a Bioxcell® extender. Semen was cooled ...
متن کاملeffect of extender and equilibration time on post thaw motility and chromatin structure of buffalo bull (bubalus bubalis) spermatozoa
objective: the aim of the present study was to investigate the effects of four equilibration times (2, 4, 8 and 16 hours) and two extenders (tris or bioxcell®) on cryopreservation of buffalo semen. materials and methods: in this experimental study, split pooled ejaculates (n=4), possessing more than 70% visual sperm motility were divided in two aliquots and diluted in bioxcell ® and tris-citric...
متن کاملeffect of different thawing rates on post-thaw viability, kinematic parameters and chromatin structure of buffalo (bubalus bubalis) spermatozoa
objective: the aim of the present study was to evaluate three thawing rates on the post thaw motility, viability and chromatin structure of buffalo semen frozen in 0.5-ml straws. materials and methods: in this experimental study semen was collected with artificial vagina (42˚c) from four buffalo bulls.split pooled ejaculates (n=4) were extended at 37˚c with a bioxcell® extender. semen was coole...
متن کاملEffect of Non-enzymatic Antioxidants in Extender on Post-thaw Quality of Buffalo (bubalus Bubalis) Bull Spermatozoa
The objective of the present study was to determine the effect of non-enzymatic antioxidants (vitamin C or E) in tris-citric acid buffer (TCA) on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa. Split pooled buffalo bull ejaculates were diluted in TCA egg yolk glycerol extender containing either vitamin C (TCAC), vitamin E (TCAE) or without antioxidant (TCAN) at 37°C. Extended s...
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ژورنال
عنوان ژورنال: Andrology
سال: 2016
ISSN: 2047-2919
DOI: 10.1111/andr.12214